CoraLite®555-conjugated G3BP1 Recombinant antibody
G3BP1 Uni-rAbTM Recombinant Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
IF/ICC
Conjugate
CoraLite®555 Fluorescent Dye
CloneNo.
7F14
验证数据展示
经过测试的应用
Positive IF/ICC detected in | sodium arsenite treated HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy G3BP1 Ready-To-Use IHC Kit. G3BP1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 80777-1-PBS
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL555-80777 targets G3BP1 in IF/ICC applications and shows reactivity with Human samples.
Tested Applications | IF/ICC Application Description |
Tested Reactivity | Human |
Immunogen | G3BP1 fusion protein Ag3728 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | GTPase activating protein (SH3 domain) binding protein 1 |
Synonyms | G3BP, G3BP 1, G3BP1, HDH VIII |
Calculated Molecular Weight | 466 aa, 52 kDa |
GenBank Accession Number | BC006997 |
Gene Symbol | G3BP1 |
Gene ID (NCBI) | 10146 |
RRID | AB_2919712 |
Conjugate | CoraLite®555 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 557 nm / 570nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q13283 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GAP SH3 Binding Protein 1 (G3BP1), also named as G3BP, is an effector of stress granule (SG) assembly. SG biology plays an important role in the pathophysiology of TDP-43 in ALS and FTLD-U. G3BP1 can be used as a marker of SG. It has been shown to function downstream of Ras and play a role in RNA metabolism, signal transduction, and proliferation. G3BP1 is a ubiquitously expressed protein that localizes to the cytoplasm in proliferating cells and to the nucleus in non-proliferating cells. G3BP1 has recently been implicated in cancer biology.
实验方案
Product Specific Protocols | |
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IF protocol for CL555 G3BP1 antibody CL555-80777 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |