• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84376-4-RR (same clone as 84376-4-PBS)

    WB analysis using 84376-4-RR (same clone as 84376-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84376-4-RR (GADD45A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84376-4-PBS in a different storage buffer formulation.

  • FC experiment of K562 using 84376-4-RR (same clone as 84376-4-PBS)

    FC experiment of K562 using 84376-4-RR (same clone as 84376-4-PBS)

    1x10^6 K562 were intracellularly stained with 0.25 ug GADD45A Recombinant antibody (84376-4-RR, Clone:241597H4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84376-4-PBS in a different storage buffer formulation.

  • FC experiment of U-2 OS using 84376-4-RR (same clone as 84376-4-PBS)

    FC experiment of U-2 OS using 84376-4-RR (same clone as 84376-4-PBS)

    1x10^6 U-2 OS were intracellularly stained with 0.25 ug GADD45A Recombinant antibody (84376-4-RR, Clone:241597H4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84376-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01247-2

    Cytometric bead array standard curve of MP01247-2

    Cytometric bead array standard curve of MP01247-2, GADD45A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84376-4-PBS. Detection antibody: 84376-1-PBS. Standard: Ag4687. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 84376-4-RR

    Affinity and Kinetic Characterization of 84376-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84376-4-RR against Human GADD45A were performed. The affinity constant is 0.769 nM.

GADD45A Recombinant antibody, PBS Only (Capture)

GADD45A Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 84376-4-PBS

产品说明书

CloneNo. 241597H4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra), Cytometric bead array, Indirect ELISA

别名

GADD45, DNA damage-inducible transcript 1 protein, DDIT-1, DDIT1, DDIT 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

84376-4-PBS targets GADD45A as part of a matched antibody pair:

MP01247-2: 84376-4-PBS capture and 84376-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 GADD45A fusion protein Ag4687 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 growth arrest and DNA-damage-inducible, alpha
别名 GADD45, DNA damage-inducible transcript 1 protein, DDIT-1, DDIT1, DDIT 1
计算分子量 165 aa, 18 kDa
观测分子量14-18 kDa, 28-36 kDa
GenBank蛋白编号BC011757
基因名称 GADD45A
Gene ID (NCBI) 1647
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP24522
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GADD45A, also named as DDIT1 and GADD45, belongs to the GADD45 family. It binds to proliferating cell nuclear antigen. GADD45A may affect PCNA interaction with some CDK (cell division protein kinase) complexes; stimulates DNA excision repair in vitro and inhibits entry of cells into S phase. GADD45A plays an essential role in active DNA demethylation during terminal osteogenic differentiation of adipose-derived mesenchymal stem cells. GADD45A has Dimer (~28-36 kDa) and Monomer (14-18 kDa) forms (PMID:11498536).