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验证数据展示

  • WB analysis using 84376-4-RR

    WB analysis using 84376-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84376-4-RR (GADD45A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of K562 using 84376-4-RR

    FC experiment of K562 using 84376-4-RR

    1x10^6 K562 were intracellularly stained with 0.25 ug GADD45A Recombinant antibody (84376-4-RR, Clone:241597H4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • FC experiment of U-2 OS using 84376-4-RR

    FC experiment of U-2 OS using 84376-4-RR

    1x10^6 U-2 OS were intracellularly stained with 0.25 ug GADD45A Recombinant antibody (84376-4-RR, Clone:241597H4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 84376-4-RR

    Affinity and Kinetic Characterization of 84376-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84376-4-RR against Human GADD45A were performed. The affinity constant is 0.769 nM.

GADD45A Recombinant antibody

GADD45A Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84376-4-RR

产品说明书

CloneNo. 241597H4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra), ELISA

别名

GADD45, DNA damage-inducible transcript 1 protein, DDIT-1, DDIT1, DDIT 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, NIH/3T3 cells
Positive FC (Intra) detected inU-2 OS, K562

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84376-4-RR targets GADD45A in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 GADD45A fusion protein Ag4687 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 growth arrest and DNA-damage-inducible, alpha
别名 GADD45, DNA damage-inducible transcript 1 protein, DDIT-1, DDIT1, DDIT 1
计算分子量 165 aa, 18 kDa
观测分子量14-18 kDa, 28-36 kDa
GenBank蛋白编号BC011757
基因名称 GADD45A
Gene ID (NCBI) 1647
RRIDAB_3671913
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP24522
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GADD45A, also named as DDIT1 and GADD45, belongs to the GADD45 family. It binds to proliferating cell nuclear antigen. GADD45A may affect PCNA interaction with some CDK (cell division protein kinase) complexes; stimulates DNA excision repair in vitro and inhibits entry of cells into S phase. GADD45A plays an essential role in active DNA demethylation during terminal osteogenic differentiation of adipose-derived mesenchymal stem cells. GADD45A has Dimer (~28-36 kDa) and Monomer (14-18 kDa) forms (PMID:11498536).

实验方案

Product Specific Protocols
WB protocol for GADD45A antibody 84376-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols