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验证数据展示

  • IF Staining of A431 using CL594-67893

    IF Staining of A431 using CL594-67893

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite®594 GARS antibody (CL594-67893, Clone: 2H9D3 ) at dilution of 1:200.

  • FC experiment of A431 using CL594-67893

    FC experiment of A431 using CL594-67893

    1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human GARS (CL594-67893, Clone:2H9D3) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated GARS Monoclonal antibody

GARS Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-67893

产品说明书

CloneNo. 2H9D3

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

GARS1, EC:2.7.7.-, DSMAV, CMT2D, Ap4A synthetase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells, A431 cell line
Planning an IHC experiment? We recommend our IHCeasy GARS Ready-To-Use IHC Kit. GARS primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67893-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67893 targets GARS in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 GARS fusion protein Ag8432 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 glycyl-tRNA synthetase
别名 GARS1, EC:2.7.7.-, DSMAV, CMT2D, Ap4A synthetase
计算分子量 739 aa, 83 kDa
观测分子量75-80 kDa
GenBank蛋白编号BC007755
基因名称 GARS
Gene ID (NCBI) 2617
RRIDAB_2920188
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP41250
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glycyl-tRNA synthetase 1 (GARS, GeneID:2617) is one of the aminoacyl-tRNA synthetases. It is essential for protein synthesis in all cells and tissues (PMID: 17035524) and charges tRNA molecules with glycine by catalyzing the ATP-dependent ligation of glycine to the 3'-end of it's cognate tRNA via the formation of an aminoacyl-adenylate intermediate (Gly-AMP) (PMID: 17544401, PMID: 28675565, PMID: 24898252) . GARS is located in cytoplasm, but also can be secreted from cells and as an extracellular protein which may influence cell signaling. Mutation of GARS causes peripheral neuropathies such as CMT2D, SMA (PMID: 26503042, PMID: 32181591) , or associates with cancer. Two transcript variants encoding different isoforms have been found for this gene. The molecular weight of isoform2 is about 78 kDa.

实验方案

Product Specific Protocols
IF protocol for CL594 GARS antibody CL594-67893Download protocol
FC protocol for CL594 GARS antibody CL594-67893Download protocol
Standard Protocols
Click here to view our Standard Protocols