验证数据展示
经过测试的应用
Positive IF/ICC detected in | MCF-7 cells |
Positive FC (Intra) detected in | Jurkat cells |
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-66400 targets GATA3 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.
Tested Applications | IF/ICC, FC (Intra) Application Description |
Tested Reactivity | human |
Immunogen | GATA3 fusion protein Ag0174 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | GATA binding protein 3 |
Synonyms | Trans-acting T-cell-specific transcription factor GATA-3, GATA-binding factor 3, GATA binding factor 3, 1B11A8 |
Calculated Molecular Weight | 443 aa, 48 kDa |
GenBank Accession Number | BC003070 |
Gene Symbol | GATA3 |
Gene ID (NCBI) | 2625 |
RRID | AB_2883327 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P23771 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GATA3 is a transcriptional factor that binds to the enhancer elements of all 4 T-cell antigen receptor genes. GATA3 is highly expressed in naive, freshly activated cells and Th2 lineage cells and thought to be necessary and sufficient for Th2 cytokine gene expression. GATA3 and TBET interfer with each other to regulate gene expression.This antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 GATA3 antibody CL488-66400 | Download protocol |
FC protocol for CL Plus 488 GATA3 antibody CL488-66400 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |