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CoraLite® Plus 488-conjugated GATA3 Monoclonal antibody

GATA3 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B11A8

Cat no : CL488-66400

Print datasheet

Synonyms

Trans-acting T-cell-specific transcription factor GATA-3, GATA-binding factor 3, GATA binding factor 3, 1B11A8

验证数据展示

  • IF Staining of MCF-7 using CL488-66400

    IF Staining of MCF-7 using CL488-66400

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 GATA3 antibody (CL488-66400, Clone: 1B11A8 ) at dilution of 1:800, CL594-Phalloidin (red).

  • FC experiment of Jurkat using CL488-66400

    FC experiment of Jurkat using CL488-66400

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human GATA3 (CL488-66400, Clone:1B11A8) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inJurkat cells
For other applications, we recommend the unconjugated version of this antibody, 66400-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66400 targets GATA3 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Immunogen GATA3 fusion protein Ag0174 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name GATA binding protein 3
Synonyms Trans-acting T-cell-specific transcription factor GATA-3, GATA-binding factor 3, GATA binding factor 3, 1B11A8
Calculated Molecular Weight 443 aa, 48 kDa
GenBank Accession NumberBC003070
Gene Symbol GATA3
Gene ID (NCBI) 2625
RRIDAB_2883327
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP23771
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GATA3 is a transcriptional factor that binds to the enhancer elements of all 4 T-cell antigen receptor genes. GATA3 is highly expressed in naive, freshly activated cells and Th2 lineage cells and thought to be necessary and sufficient for Th2 cytokine gene expression. GATA3 and TBET interfer with each other to regulate gene expression.This antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 GATA3 antibody CL488-66400Download protocol
FC protocol for CL Plus 488 GATA3 antibody CL488-66400Download protocol
Standard Protocols
Click here to view our Standard Protocols