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验证数据展示

  • IF Staining of human breast cancer using CL594-66400

    IF Staining of human breast cancer using CL594-66400

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CL594-66400 (GATA3 antibody) at dilution of 1:50.

  • IF Staining of MCF-7 using CL594-66400

    IF Staining of MCF-7 using CL594-66400

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite®594 GATA3 antibody (CL594-66400, Clone: 1B11A8 ) at dilution of 1:800, CL488-Phalloidin (green).

  • IF Staining of human breast cancer using CL594-66400

    IF Staining of human breast cancer using CL594-66400

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CL594-66400 (GATA3 antibody) at dilution of 1:50.

CoraLite®594-conjugated GATA3 Monoclonal antibody

GATA3 Monoclonal Antibody for IF/ICC, IF-P
Cat No. CL594-66400

产品说明书

CloneNo. 1B11A8

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

IF/ICC, IF-P

别名

Trans-acting T-cell-specific transcription factor GATA-3, GATA-binding factor 3, GATA binding factor 3, 1B11A8

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman breast cancer tissue
Positive IF/ICC detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 66400-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66400 targets GATA3 in IF/ICC, IF-P applications and shows reactivity with human samples.

经测试应用 IF/ICC, IF-P Application Description
经测试反应性 human
免疫原 GATA3 fusion protein Ag0174 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 GATA binding protein 3
别名 Trans-acting T-cell-specific transcription factor GATA-3, GATA-binding factor 3, GATA binding factor 3, 1B11A8
计算分子量 443 aa, 48 kDa
GenBank蛋白编号BC003070
基因名称 GATA3
Gene ID (NCBI) 2625
RRIDAB_2883576
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP23771
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GATA3 is a transcriptional factor that binds to the enhancer elements of all 4 T-cell antigen receptor genes. GATA3 is highly expressed in naive, freshly activated cells and Th2 lineage cells and thought to be necessary and sufficient for Th2 cytokine gene expression. GATA3 and TBET interfer with each other to regulate gene expression. This antibody is CL594(Ex/Em 593 nm/614 nm) conjugated.

实验方案

Product Specific Protocols
IF protocol for CL594 GATA3 antibody CL594-66400Download protocol
Standard Protocols
Click here to view our Standard Protocols