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验证数据展示

  • WB analysis using 84264-6-RR (same clone as 84264-6-PBS)

    WB analysis using 84264-6-RR (same clone as 84264-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84264-6-RR (GBP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84264-6-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 84264-6-RR (same clone as 84264-6-PBS)

    IHC staining of human tonsillitis using 84264-6-RR (same clone as 84264-6-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84264-6-RR (GBP2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84264-6-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 84264-6-RR (same clone as 84264-6-PBS)

    IF Staining of HeLa using 84264-6-RR (same clone as 84264-6-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GBP2 antibody (84264-6-RR, Clone: 241580C12 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84264-6-PBS in a different storage buffer formulation.

  • FC experiment of A549 using 84264-6-RR (same clone as 84264-6-PBS)

    FC experiment of A549 using 84264-6-RR (same clone as 84264-6-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.2 ug GBP2 Recombinant antibody (84264-6-RR, Clone:241580C12) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005)(red), or 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 84264-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84264-6-RR

    Affinity and Kinetic Characterization of 84264-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84264-6-RR against Human GBP2 were performed. The affinity constant is below 1 pM.

GBP2 Recombinant monoclonal antibody, PBS Only

GBP2 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84264-6-PBS

产品说明书

CloneNo. 241580C12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

241580C12, EC:3.6.5.-, GBP 2, GBP-2, GTP-binding protein 2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

84264-6-PBS targets GBP2 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag2425

Product name: Recombinant human GBP2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 131-481 aa of BC022272

Sequence: MGTINQQAMDQLHYVTELTDRIKANSSPGNNSVDDSADFVSFFPAFVWTLRDFTLELEVDGEPITADDYLELSLKLRKGTDKKSKSFNDPRLCIRKFFPKRKCFVFDWPAPKKYLAHLEQLKEEELNPDFIEQVAEFCSYILSHSNVKTLSGGIPVNGPRLESLVLTYVNAISSGDLPCMENAVLALAQIENSAAVEKAIAHYEQQMGQKVQLPTETLQELLDLHRDSEREAIEVFMKNSFKDVDQMFQRKLGAQLEARRDDFCKQNSKASSDCCMALLQDIFGPLEEDVKQGTFSKPGGYRLFTQKLQELKNKYYQVPRKGIQAKEVLKKYLESKEDVADALLQTDQSLS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 guanylate binding protein 2, interferon-inducible
别名 241580C12, EC:3.6.5.-, GBP 2, GBP-2, GTP-binding protein 2
计算分子量 591 aa, 67 kDa
观测分子量67 kDa
GenBank蛋白编号BC022272
基因名称 GBP2
Gene ID (NCBI) 2634
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP32456
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GBP2 is a member of the GTPase family and is crucial to host immunity against pathogens. GBP2 is also used as a marker of interferon (IFN) responsiveness because it is one of the most highly expressed genes after IFN-γ stimulation. Increased GBP2 expression is also associated with a better prognosis in breast cancer and may have a role in T-cell defense against breast cancer. Interestingly, GBP2 expression in NIH/3T3 fibroblasts inhibited Rac activation and matrix metalloproteinase-9 expression, suggesting a possible role for GBP2 in regulating cancer metastasis. The molecular weight of GBP2 is 67 kDa. (PMID: 29072687, 35383115)

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