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GBP2 Recombinant antibody, PBS Only

GBP2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241580C12

Cat no : 84264-6-PBS

Print datasheet

Synonyms

Guanine nucleotide-binding protein 2, GTP-binding protein 2, GBP-2, GBP 2, EC:3.6.5.-

验证数据展示

  • WB analysis using 84264-6-RR (same clone as 84264-6-PBS)

    WB analysis using 84264-6-RR (same clone as 84264-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84264-6-RR (GBP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84264-6-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 84264-6-RR (same clone as 84264-6-PBS)

    IF Staining of HeLa using 84264-6-RR (same clone as 84264-6-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GBP2 antibody (84264-6-RR, Clone: 241580C12 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84264-6-PBS in a different storage buffer formulation.

  • FC experiment of A549 using 84264-6-RR (same clone as 84264-6-PBS)

    FC experiment of A549 using 84264-6-RR (same clone as 84264-6-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.2 ug GBP2 Recombinant antibody (84264-6-RR, Clone:241580C12) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005)(red), or 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84264-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84264-6-RR

    Affinity and Kinetic Characterization of 84264-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84264-6-RR against Human GBP2 were performed. The affinity constant is below 1 pM.

产品信息

The immunogen of 84264-6-PBS is GBP2 Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human
Immunogen GBP2 fusion protein Ag2425 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name guanylate binding protein 2, interferon-inducible
Synonyms Guanine nucleotide-binding protein 2, GTP-binding protein 2, GBP-2, GBP 2, EC:3.6.5.-
Calculated Molecular Weight 591 aa, 67 kDa
Observed Molecular Weight67 kDa
GenBank Accession NumberBC022272
Gene Symbol GBP2
Gene ID (NCBI) 2634
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP32456
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GBP2 is a member of the GTPase family and is crucial to host immunity against pathogens. GBP2 is also used as a marker of interferon (IFN) responsiveness because it is one of the most highly expressed genes after IFN-γ stimulation. Increased GBP2 expression is also associated with a better prognosis in breast cancer and may have a role in T-cell defense against breast cancer. Interestingly, GBP2 expression in NIH/3T3 fibroblasts inhibited Rac activation and matrix metalloproteinase-9 expression, suggesting a possible role for GBP2 in regulating cancer metastasis. The molecular weight of GBP2 is 67 kDa. (PMID: 29072687, 35383115)