验证数据展示
经过测试的应用
| Positive WB detected in | U-937 cells, Jurkat cells |
| Positive IP detected in | U-937 cells |
| Positive IHC detected in | rat spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | MCF-7 cells |
Planning an IHC experiment? We recommend our IHCeasy GBP5 Ready-To-Use IHC Kit. GBP5 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13220-1-AP targets GBP5 in WB, IHC, IP, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, rat |
| 文献引用反应性 | human, rat |
| 免疫原 | GBP5 fusion protein Ag4103 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | guanylate binding protein 5 |
| 别名 | Guanylate-binding protein 5, Guanine nucleotide-binding protein 5, GTP-binding protein 5, GBP-TA antigen, GBP-5 |
| 计算分子量 | 586 aa, 67 kDa |
| 观测分子量 | 65 kDa, 55 kDa |
| GenBank蛋白编号 | BC031639 |
| 基因名称 | GBP5 |
| Gene ID (NCBI) | 115362 |
| RRID | AB_2109348 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96PP8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Guanylate binding protein (GBP) 5 belongs to the GBP family, which is involved in important cellular processes, including signal transduction, translation, vesicle trafficking, and exocytosis. GBP5 was reported to be a critical cellular factor in inflammasome assembly. Furthermore, GBP5 has been reported to induce inflammatory cytokines, including IL-1 beta and IL-18, through the activation of NLRP3 and AIM2. GBP5 encodes two isoforms: GBP-5a/b (67 kDa) and GBP-5ta (55 kDa)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GBP5 antibody 13220-1-AP | Download protocol |
| IHC protocol for GBP5 antibody 13220-1-AP | Download protocol |
| IP protocol for GBP5 antibody 13220-1-AP | Download protocol |
| FC protocol for GBP5 antibody 13220-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nature Ubiquitination and degradation of GBPs by a Shigella effector to suppress host defence. | ||
Nature Caspase-11 activation requires lysis of pathogen-containing vacuoles by IFN-induced GTPases. | ||
Cell IRGB10 Liberates Bacterial Ligands for Sensing by the AIM2 and Caspase-11-NLRP3 Inflammasomes. | ||
Nat Immunol The transcription factor IRF1 and guanylate-binding proteins target activation of the AIM2 inflammasome by Francisella infection. | ||
Nat Immunol Guanylate-binding proteins promote activation of the AIM2 inflammasome during infection with Francisella novicida.
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