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验证数据展示

  • FC experiment of MCF-7 using CL647-13220

    FC experiment of MCF-7 using CL647-13220

    1x10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 647-conjugated GBP5 Polyclonal antibody (CL647-13220)(red), or 0.4 ug CoraLite® Plus 647-conjugated Rabbit IgG control Rabbit PolyAb (CL647-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 647-conjugated GBP5 Polyclonal antibody

GBP5 Polyclonal Antibody for FC (Intra)
Cat No. CL647-13220

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC (Intra) and More (2)

别名

Guanylate-binding protein 5, Guanine nucleotide-binding protein 5, GTP-binding protein 5, GBP-TA antigen, GBP-5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy GBP5 Ready-To-Use IHC Kit. GBP5 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 13220-1-AP

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-13220 targets GBP5 in WB, IF, FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
文献引用应用WB, IF
经测试反应性 human
免疫原 GBP5 fusion protein Ag4103 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 guanylate binding protein 5
别名 Guanylate-binding protein 5, Guanine nucleotide-binding protein 5, GTP-binding protein 5, GBP-TA antigen, GBP-5
计算分子量 586 aa, 67 kDa
观测分子量 65 kDa, 55 kDa
GenBank蛋白编号BC031639
基因名称 GBP5
Gene ID (NCBI) 115362
RRIDAB_2934881
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96PP8
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Guanylate binding protein (GBP) 5 belongs to the GBP family, which is involved in important cellular processes, including signal transduction, translation, vesicle trafficking, and exocytosis. GBP5 was reported to be a critical cellular factor in inflammasome assembly. Furthermore, GBP5 has been reported to induce inflammatory cytokines, including IL-1 beta and IL-18, through the activation of NLRP3 and AIM2. GBP5 encodes two isoforms: GBP-5a/b (67 kDa) and GBP-5ta (55 kDa)

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 GBP5 antibody CL647-13220Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB,IF

Ecotoxicol Environ Saf

Treatment of silicosis with quercetin depolarizing macrophages via inhibition of mitochondrial damage-associated pyroptosis

Authors - Chuan-Yong Xiao
View Article