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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated GBP5 Polyclonal antibody

GBP5 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra) and More (2)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-13220

Print datasheet

Synonyms

Guanylate-binding protein 5, Guanine nucleotide-binding protein 5, GTP-binding protein 5, GBP-TA antigen, GBP-5

验证数据展示

  • FC experiment of MCF-7 using CL647-13220

    FC experiment of MCF-7 using CL647-13220

    1x10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 647-conjugated GBP5 Polyclonal antibody (CL647-13220)(red), or 0.4 ug CoraLite® Plus 647-conjugated Rabbit IgG control Rabbit PolyAb (CL647-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy GBP5 Ready-To-Use IHC Kit. GBP5 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 13220-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-13220 targets GBP5 in WB, IF, FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra) Application Description
Cited ApplicationsWB, IF
Tested Reactivity human
Immunogen GBP5 fusion protein Ag4103 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name guanylate binding protein 5
Synonyms Guanylate-binding protein 5, Guanine nucleotide-binding protein 5, GTP-binding protein 5, GBP-TA antigen, GBP-5
Calculated Molecular Weight 586 aa, 67 kDa
Observed Molecular Weight 65 kDa, 55 kDa
GenBank Accession NumberBC031639
Gene Symbol GBP5
Gene ID (NCBI) 115362
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96PP8
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Guanylate binding protein (GBP) 5 belongs to the GBP family, which is involved in important cellular processes, including signal transduction, translation, vesicle trafficking, and exocytosis. GBP5 was reported to be a critical cellular factor in inflammasome assembly. Furthermore, GBP5 has been reported to induce inflammatory cytokines, including IL-1 beta and IL-18, through the activation of NLRP3 and AIM2. GBP5 encodes two isoforms: GBP-5a/b (67 kDa) and GBP-5ta (55 kDa)

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 GBP5 antibody CL647-13220Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB,IF

Ecotoxicol Environ Saf

Treatment of silicosis with quercetin depolarizing macrophages via inhibition of mitochondrial damage-associated pyroptosis

Authors - Chuan-Yong Xiao
View Article