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验证数据展示

  • FC experiment of MCF-7 using CL488-13220

    FC experiment of MCF-7 using CL488-13220

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human GBP5 (CL488-13220) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of MCF-7 using CL488-13220

    IF Staining of MCF-7 using CL488-13220

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 GBP5 antibody (CL488-13220) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated GBP5 Polyclonal antibody

GBP5 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-13220

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

Guanylate-binding protein 5, Guanine nucleotide-binding protein 5, GTP-binding protein 5, GBP-TA antigen, GBP-5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy GBP5 Ready-To-Use IHC Kit. GBP5 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 13220-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-13220 targets GBP5 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 GBP5 fusion protein Ag4103 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 guanylate binding protein 5
别名 Guanylate-binding protein 5, Guanine nucleotide-binding protein 5, GTP-binding protein 5, GBP-TA antigen, GBP-5
计算分子量 586 aa, 67 kDa
观测分子量 65 kDa, 55 kDa
GenBank蛋白编号BC031639
基因名称 GBP5
Gene ID (NCBI) 115362
RRIDAB_2934405
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96PP8
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Guanylate binding protein (GBP) 5 belongs to the GBP family, which is involved in important cellular processes, including signal transduction, translation, vesicle trafficking, and exocytosis. GBP5 was reported to be a critical cellular factor in inflammasome assembly. Furthermore, GBP5 has been reported to induce inflammatory cytokines, including IL-1 beta and IL-18, through the activation of NLRP3 and AIM2. GBP5 encodes two isoforms: GBP-5a/b (67 kDa) and GBP-5ta (55 kDa)1

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 GBP5 antibody CL488-13220Download protocol
Standard Protocols
Click here to view our Standard Protocols