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验证数据展示

  • IF Staining of HeLa using CL488-60078

    IF Staining of HeLa using CL488-60078

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 GDI2 antibody (CL488-60078, Clone: 4B8F3 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated GDI2 Monoclonal antibody

GDI2 Monoclonal Antibody for IF/ICC
Cat No. CL488-60078

产品说明书

CloneNo. 4B8F3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

IF/ICC

别名

Rab GDI β, Rab GDI beta, GDP dissociation inhibitor 2, GDI-2, GDI 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy GDI2 Ready-To-Use IHC Kit. GDI2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60078-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-60078 targets GDI2 in IF/ICC applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse
免疫原 GDI2 fusion protein Ag4613 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 GDP dissociation inhibitor 2
别名 Rab GDI β, Rab GDI beta, GDP dissociation inhibitor 2, GDI-2, GDI 2
计算分子量 47 kDa
观测分子量 46 kDa
GenBank蛋白编号BC005145
基因名称 GDI2
Gene ID (NCBI) 2665
RRIDAB_2883097
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP50395
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GDP dissociation inhibitors (GDIs) are proteins that regulate the GDP-GTP exchange reaction of members of the rab family, GDIs can bind and release GDP-bound Rab proteins from membranes. Two GDI proteins towards different Rab proteins have been identified. GDI1 interacts with almost all of the Rab proteins, while GDI2 interacts with Rabll but not Rab3A. GDI2 distributes ubiquitously, displaying a membrane bound location in perinuclear regions of cells. GDI-2 was thought to be involved in cellular response to insulin. It electrophoreses as a 46kd protein in SDS-PAGE. (PMID: 7929030; PMID: 19570034). This antibody can bind both GDIs for the close sequences.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 GDI2 antibody CL488-60078Download protocol
Standard Protocols
Click here to view our Standard Protocols