CoraLite®594-conjugated GDI2 Monoclonal antibody
GDI2 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse
Applications
IF/ICC
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
4B8F3
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy GDI2 Ready-To-Use IHC Kit. GDI2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60078-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-60078 targets GDI2 in IF/ICC applications and shows reactivity with human, mouse samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | human, mouse |
| Immunogen | GDI2 fusion protein Ag4613 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | GDP dissociation inhibitor 2 |
| Synonyms | Rab GDI β, Rab GDI beta, GDP dissociation inhibitor 2, GDI-2, GDI 2 |
| Calculated Molecular Weight | 47 kDa |
| Observed Molecular Weight | 46 kDa |
| GenBank Accession Number | BC005145 |
| Gene Symbol | GDI2 |
| Gene ID (NCBI) | 2665 |
| RRID | AB_2883417 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P50395 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GDP dissociation inhibitors (GDIs) are proteins that regulate the GDP-GTP exchange reaction of members of the rab family, GDIs can bind and release GDP-bound Rab proteins from membranes. Two GDI proteins towards different Rab proteins have been identified. GDI1 interacts with almost all of the Rab proteins, while GDI2 interacts with Rabll but not Rab3A. GDI2 distributes ubiquitously, displaying a membrane bound location in perinuclear regions of cells. GDI-2 was thought to be involved in cellular response to insulin. It electrophoreses as a 46kd protein in SDS-PAGE. (PMID: 7929030; PMID: 19570034). This antibody can bind both GDIs for the close sequences.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 GDI2 antibody CL594-60078 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |