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验证数据展示

  • WB analysis using 84486-2-RR (same clone as 84486-2-PBS)

    WB analysis using 84486-2-RR (same clone as 84486-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84486-2-RR (GINS2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84486-2-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 84486-2-RR (same clone as 84486-2-PBS)

    IF Staining of HeLa using 84486-2-RR (same clone as 84486-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GINS2 antibody (84486-2-RR, Clone: 241870E9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84486-2-PBS in a different storage buffer formulation.

  • FC experiment of Jurkat using 84486-2-RR (same clone as 84486-2-PBS)

    FC experiment of Jurkat using 84486-2-RR (same clone as 84486-2-PBS)

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug GINS2 Recombinant antibody (84486-2-RR, Clone:241870E9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 84486-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01325-1

    Cytometric bead array standard curve of MP01325-1

    Cytometric bead array standard curve of MP01325-1, GINS2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84486-2-PBS. Detection antibody: 84486-1-PBS. Standard: Ag9180. Range: 0.313-20 ng/mL.

  • Cytometric bead array standard curve of MP01325-2

    Cytometric bead array standard curve of MP01325-2

    Cytometric bead array standard curve of MP01325-2, GINS2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84486-2-PBS. Detection antibody: 84486-3-PBS. Standard: Ag9180. Range: 0.313-20 ng/mL.

  • Affinity and Kinetic Characterization of 84486-2-RR

    Affinity and Kinetic Characterization of 84486-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84486-2-RR against Human GINS2 were performed. The affinity constant is 0.82 nM.

GINS2 Recombinant antibody, PBS Only (Capture)

GINS2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 84486-2-PBS

产品说明书

CloneNo. 241870E9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

PSF2, Pfs2, HSPC037, GINS 2, DC5

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84486-2-PBS targets GINS2 as part of a matched antibody pair:

MP01325-1: 84486-2-PBS capture and 84486-1-PBS detection (validated in Cytometric bead array)

MP01325-2: 84486-2-PBS capture and 84486-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 GINS2 fusion protein Ag9180 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 GINS complex subunit 2 (Psf2 homolog)
别名 PSF2, Pfs2, HSPC037, GINS 2, DC5
计算分子量 185 aa, 21 kDa
观测分子量20-25 kDa
GenBank蛋白编号BC010164
基因名称 GINS2
Gene ID (NCBI) 51659
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y248
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GINS2, also named as DNA replication complex GINS protein PSF2, CGI-122, DC5, or HSPC037, is a 185 amino acid protein, which belongs to the GINS2/PSF2 family. The GINS complex plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS complex seems to bind preferentially to single-stranded DNA. GINS2 is a part of the the GINS complex, an integral component of the replicative helicase machinery that facilitates progression of the replisome complex.