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验证数据展示

  • WB analysis using 84486-2-RR

    WB analysis using 84486-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84486-2-RR (GINS2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 84486-2-RR

    IF Staining of HeLa using 84486-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GINS2 antibody (84486-2-RR, Clone: 241870E9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of Jurkat using 84486-2-RR

    FC experiment of Jurkat using 84486-2-RR

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug GINS2 Recombinant antibody (84486-2-RR, Clone:241870E9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 84486-2-RR

    Affinity and Kinetic Characterization of 84486-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84486-2-RR against Human GINS2 were performed. The affinity constant is 0.82 nM.

GINS2 Recombinant antibody

GINS2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 84486-2-RR

产品说明书

CloneNo. 241870E9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

PSF2, Pfs2, HSPC037, GINS 2, DC5

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inJurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84486-2-RR targets GINS2 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 GINS2 fusion protein Ag9180 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 GINS complex subunit 2 (Psf2 homolog)
别名 PSF2, Pfs2, HSPC037, GINS 2, DC5
计算分子量 185 aa, 21 kDa
观测分子量20-25 kDa
GenBank蛋白编号BC010164
基因名称 GINS2
Gene ID (NCBI) 51659
RRIDAB_3672003
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y248
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GINS2, also named as DNA replication complex GINS protein PSF2, CGI-122, DC5, or HSPC037, is a 185 amino acid protein, which belongs to the GINS2/PSF2 family. The GINS complex plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS complex seems to bind preferentially to single-stranded DNA. GINS2 is a part of the the GINS complex, an integral component of the replicative helicase machinery that facilitates progression of the replisome complex.

实验方案

Product Specific Protocols
WB protocol for GINS2 antibody 84486-2-RRDownload protocol
IF protocol for GINS2 antibody 84486-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols