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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated GMAP-210 Polyclonal antibody

GMAP-210 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-26456

Print datasheet

Synonyms

CEV14, GMAP 210, GMAP-210, TR interacting protein 11, TRIP 11, TRIP11, TRIP230

验证数据展示

  • IF Staining of HeLa using CL488-26456

    IF Staining of HeLa using CL488-26456

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 GMAP-210 antibody (CL488-26456) at dilution of 1:200, CL594-Phalloidin (red).

经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy TRIP11 Ready-To-Use IHC Kit. TRIP11 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 26456-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-26456 targets GMAP-210 in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen GMAP-210 fusion protein Ag13867 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name thyroid hormone receptor interactor 11
Synonyms CEV14, GMAP 210, GMAP-210, TR interacting protein 11, TRIP 11, TRIP11, TRIP230
Calculated Molecular Weight 1979 aa, 228 kDa
Observed Molecular Weight 230 kDa
GenBank Accession NumberBC002656
Gene Symbol TRIP11
Gene ID (NCBI) 9321
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15643
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Golgi microtubule-associated protein 210 (GMAP-210), also referred to as CEV14, Trip11 or Trip230, is a peripheral Golgi protein that localizes to the cis-Golgi network. GMAP-210 is a 1,978 amino acid coiled-coil member of the golgin family of proteins. Microtubule ends bind to GMAP-210 which functions to link the cis-Golgi network to the minus ends of centrosome-nucleated microtubules. This interaction may be essential for the proper morphology and structural maintenance of the Golgi apparatus. GMAP-210 also associates with thyroid hormone receptor. Overexpression of GMAP-210 disrupts the micro-tubule network and causes a significant enlargement and fragmentation of the Golgi apparatus; it also blocks anterograde and retrograde transport between the ER and the Golgi apparatus.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 GMAP-210 antibody CL488-26456Download protocol
Standard Protocols
Click here to view our Standard Protocols