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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated GMAP-210 Polyclonal antibody

GMAP-210 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-26456

Print datasheet

Synonyms

CEV14, GMAP 210, GMAP-210, TR interacting protein 11, TRIP 11, TRIP11, TRIP230

验证数据展示

  • IF Staining of HeLa using CL488-26456

    IF Staining of HeLa using CL488-26456

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 GMAP-210 antibody (CL488-26456) at dilution of 1:200, CL594-Phalloidin (red).

经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy TRIP11 Ready-To-Use IHC Kit. TRIP11 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 26456-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-26456 targets GMAP-210 in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen GMAP-210 fusion protein Ag13867 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name thyroid hormone receptor interactor 11
Synonyms CEV14, GMAP 210, GMAP-210, TR interacting protein 11, TRIP 11, TRIP11, TRIP230
Calculated Molecular Weight 1979 aa, 228 kDa
Observed Molecular Weight 230 kDa
GenBank Accession NumberBC002656
Gene Symbol TRIP11
Gene ID (NCBI) 9321
RRIDAB_3672798
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15643
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Golgi microtubule-associated protein 210 (GMAP-210), also referred to as CEV14, Trip11 or Trip230, is a peripheral Golgi protein that localizes to the cis-Golgi network. GMAP-210 is a 1,978 amino acid coiled-coil member of the golgin family of proteins. Microtubule ends bind to GMAP-210 which functions to link the cis-Golgi network to the minus ends of centrosome-nucleated microtubules. This interaction may be essential for the proper morphology and structural maintenance of the Golgi apparatus. GMAP-210 also associates with thyroid hormone receptor. Overexpression of GMAP-210 disrupts the micro-tubule network and causes a significant enlargement and fragmentation of the Golgi apparatus; it also blocks anterograde and retrograde transport between the ER and the Golgi apparatus.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 GMAP-210 antibody CL488-26456Download protocol
Standard Protocols
Click here to view our Standard Protocols