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GNAI2 Monoclonal antibody

GNAI2 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3F6H5

Cat no : 67007-1-Ig

Print datasheet

Synonyms

GIP, GNAI2, GNAI2B, H_LUCA15.1, H_LUCA16.1

验证数据展示

  • WB analysis using 67007-1-Ig

    WB analysis using 67007-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67007-1-Ig (GNAI2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 67007-1-Ig

    IF Staining of A431 using 67007-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using 67007-1-Ig (GNAI2 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inHL-60 cells, U-937 cells, C6 cells, RAW 264.7 cells
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy GNAI2 Ready-To-Use IHC Kit. GNAI2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

67007-1-Ig targets GNAI2 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human, mouse, rat
Cited Reactivityrabbit
Immunogen GNAI2 fusion protein Ag28560 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2
Synonyms GIP, GNAI2, GNAI2B, H_LUCA15.1, H_LUCA16.1
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberBC012138
Gene Symbol GNAI2
Gene ID (NCBI) 2771
RRIDAB_2882324
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP04899
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GNAI2, also named as GNAI2B, belongs to the G-alpha family. G(i/o/t/z) subfamily. Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. GNAI2 is 93% homolog to GNAI1, 94% to GNAI3, 85% to GNAT3, 82% to GNAT2.

实验方案

Product Specific Protocols
WB protocol for GNAI2 antibody 67007-1-IgDownload protocol
IF protocol for GNAI2 antibody 67007-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
rabbitWB

Genes (Basel)

GNAI2 Promotes Proliferation and Decreases Apoptosis in Rabbit Melanocytes.

Authors - Shuaishuai Hu
View Article