验证数据展示
经过测试的应用
| Positive IF/ICC detected in | A431 cells |
Planning an IHC experiment? We recommend our IHCeasy GNAI2 Ready-To-Use IHC Kit. GNAI2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67007-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67007 targets GNAI2 in IF/ICC applications and shows reactivity with Human, mouse, rat samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | Human, mouse, rat |
| 免疫原 | GNAI2 fusion protein Ag28560 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2 |
| 别名 | GIP, GNAI2, GNAI2B, H_LUCA15.1, H_LUCA16.1 |
| 计算分子量 | 41 kDa |
| 观测分子量 | 35-40 kDa |
| GenBank蛋白编号 | BC012138 |
| 基因名称 | GNAI2 |
| Gene ID (NCBI) | 2771 |
| RRID | AB_2934511 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P04899 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GNAI2, also named as GNAI2B, belongs to the G-alpha family. G(i/o/t/z) subfamily. Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. GNAI2 is 93% homolog to GNAI1, 94% to GNAI3, 85% to GNAT3, 82% to GNAT2.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 GNAI2 antibody CL488-67007 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |