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验证数据展示

  • IF Staining of A431 using CL594-67007

    IF Staining of A431 using CL594-67007

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite®594 GNAI2 antibody (CL594-67007, Clone: 3F6H5 ) at dilution of 1:200.

  • FC experiment of U-937 using CL594-67007

    FC experiment of U-937 using CL594-67007

    1x10^6 U-937 cells were intracellularly stained with 0.4 ug CoraLite®594-conjugated GNAI2 Monoclonal antibody (CL594-67007, Clone:3F6H5)(red), or 0.4 ug CoraLite®594 Mouse IgG1 Isotype Control (1F8D3) (CL594-66360-1, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite®594-conjugated GNAI2 Monoclonal antibody

GNAI2 Monoclonal Antibody for IF/ICC
Cat No. CL594-67007

产品说明书

CloneNo. 3F6H5

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

IF/ICC

别名

H_LUCA15.1, Guanine nucleotide-binding protein G(i) subunit alpha-2, GNAI2B, GIP, Adenylate cyclase-inhibiting G alpha protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inU-937 cells
Planning an IHC experiment? We recommend our IHCeasy GNAI2 Ready-To-Use IHC Kit. GNAI2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67007-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67007 targets GNAI2 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原 GNAI2 fusion protein Ag28560 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2
别名 H_LUCA15.1, Guanine nucleotide-binding protein G(i) subunit alpha-2, GNAI2B, GIP, Adenylate cyclase-inhibiting G alpha protein
计算分子量 41 kDa
观测分子量 35-40 kDa
GenBank蛋白编号BC012138
基因名称 GNAI2
Gene ID (NCBI) 2771
RRIDAB_3673512
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP04899
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GNAI2, also named as GNAI2B, belongs to the G-alpha family. G(i/o/t/z) subfamily. Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. GNAI2 is 93% homolog to GNAI1, 94% to GNAI3, 85% to GNAT3, 82% to GNAT2.

实验方案

Product Specific Protocols
IF protocol for CL594 GNAI2 antibody CL594-67007Download protocol
Standard Protocols
Click here to view our Standard Protocols