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验证数据展示

  • WB analysis using 68156-1-Ig

    WB analysis using 68156-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68156-1-Ig (GNAI3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 68156-1-Ig

    IF Staining of HeLa using 68156-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GNAI3 antibody (68156-1-Ig, Clone: 1A9B6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

GNAI3 Monoclonal antibody

GNAI3 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68156-1-Ig

产品说明书

CloneNo. 1A9B6

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat

应用

WB, IF/ICC, ELISA

别名

87U6, FLJ26559, G(i) alpha 3, GNAI3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, LNCaP cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy GNAI3 Ready-To-Use IHC Kit. GNAI3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68156-1-Ig targets GNAI3 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原 GNAI3 fusion protein Ag32662 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3
别名 87U6, FLJ26559, G(i) alpha 3, GNAI3
计算分子量 40 kDa
观测分子量~40 kDa
GenBank蛋白编号BC025285
基因名称 GNAI3
Gene ID (NCBI) 2773
RRIDAB_2923678
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP08754
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GNAI3 is a member of the Gi group proteins, which belong to the Gα protein family. GNAI3 is involved in several critical biological processes and regulates many cellular activities, including proliferation, differentiation, apoptosis, and migration. For example, GNAI3 can regulate a class of K+ channels in response to hormone and neurotransmitter signals. In addition to its function as a downstream signal pathway switch for receptors on the plasma membrane, GNAI3 has also been shown to localize to the centrosome and affect cytokinesis. These indicate that GNAI3 has important receptor-independent functions. The molecular weight of GNAI3 is 40 kDa. (PMID: 25444921, 34803495)

实验方案

Product Specific Protocols
WB protocol for GNAI3 antibody 68156-1-IgDownload protocol
IF protocol for GNAI3 antibody 68156-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols