验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-68156 targets GNAI3 in IF/ICC applications and shows reactivity with Human, Mouse, Rat samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | Human, Mouse, Rat |
| 免疫原 | GNAI3 fusion protein Ag32662 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3 |
| 别名 | 87U6, FLJ26559, G(i) alpha 3, GNAI3 |
| 计算分子量 | 40 kDa |
| 观测分子量 | ~40 kDa |
| GenBank蛋白编号 | BC025285 |
| 基因名称 | GNAI3 |
| Gene ID (NCBI) | 2773 |
| RRID | AB_3084422 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P08754 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GNAI3 is a member of the Gi group proteins, which belong to the Gα protein family. GNAI3 is involved in several critical biological processes and regulates many cellular activities, including proliferation, differentiation, apoptosis, and migration. For example, GNAI3 can regulate a class of K+ channels in response to hormone and neurotransmitter signals. In addition to its function as a downstream signal pathway switch for receptors on the plasma membrane, GNAI3 has also been shown to localize to the centrosome and affect cytokinesis. These indicate that GNAI3 has important receptor-independent functions. The molecular weight of GNAI3 is 40 kDa. (PMID: 25444921, 34803495)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 GNAI3 antibody CL488-68156 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |