CoraLite® Plus 488-conjugated GNAI3 Monoclonal antibody
GNAI3 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1A9B6
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy GNAI3 Ready-To-Use IHC Kit. GNAI3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68156-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-68156 targets GNAI3 in IF/ICC applications and shows reactivity with Human, Mouse, Rat samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | Human, Mouse, Rat |
| Immunogen | GNAI3 fusion protein Ag32662 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3 |
| Synonyms | 87U6, FLJ26559, G(i) alpha 3, GNAI3 |
| Calculated Molecular Weight | 40 kDa |
| Observed Molecular Weight | ~40 kDa |
| GenBank Accession Number | BC025285 |
| Gene Symbol | GNAI3 |
| Gene ID (NCBI) | 2773 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P08754 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GNAI3 is a member of the Gi group proteins, which belong to the Gα protein family. GNAI3 is involved in several critical biological processes and regulates many cellular activities, including proliferation, differentiation, apoptosis, and migration. For example, GNAI3 can regulate a class of K+ channels in response to hormone and neurotransmitter signals. In addition to its function as a downstream signal pathway switch for receptors on the plasma membrane, GNAI3 has also been shown to localize to the centrosome and affect cytokinesis. These indicate that GNAI3 has important receptor-independent functions. The molecular weight of GNAI3 is 40 kDa. (PMID: 25444921, 34803495)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 GNAI3 antibody CL488-68156 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |