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CoraLite® Plus 488-conjugated GNAT1 Polyclonal antibody

GNAT1 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-55167

Print datasheet

Synonyms

Transducin alpha-1 chain, Transducin alpha 1 chain, Guanine nucleotide-binding protein G(t) subunit alpha-1, GNATR, GBT1

验证数据展示

  • FC experiment of HeLa using CL488-55167

    FC experiment of HeLa using CL488-55167

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human GNAT1 (CL488-55167) (red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 55167-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-55167 targets GNAT1 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name guanine nucleotide binding protein (G protein), alpha transducing activity polypeptide 1
Synonyms Transducin alpha-1 chain, Transducin alpha 1 chain, Guanine nucleotide-binding protein G(t) subunit alpha-1, GNATR, GBT1
Calculated Molecular Weight 40 kDa
Observed Molecular Weight35-40 kDa
GenBank Accession NumberNM_000172
Gene Symbol GNAT1
Gene ID (NCBI) 2779
RRIDAB_3672856
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP11488
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GNAT1, also named as GNATR, belongs to the G-alpha family and G(i/o/t/z) subfamily. Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Transducin is an amplifier and one of the transducers of a visual impulse that performs the coupling between rhodopsin and cGMP-phosphodiesterase. Defects in GNAT1 are the cause of congenital stationary night blindness autosomal dominant type 3 (CSNBAD3). This antibody is specific to GNAT1.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 GNAT1 antibody CL488-55167Download protocol
Standard Protocols
Click here to view our Standard Protocols