验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | HeLa cells |
For other applications, we recommend the unconjugated version of this antibody, 55167-1-AP
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-55167 targets GNAT1 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | FC (Intra) Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | guanine nucleotide binding protein (G protein), alpha transducing activity polypeptide 1 |
| 别名 | Transducin alpha-1 chain, Transducin alpha 1 chain, Guanine nucleotide-binding protein G(t) subunit alpha-1, GNATR, GBT1 |
| 计算分子量 | 40 kDa |
| 观测分子量 | 35-40 kDa |
| GenBank蛋白编号 | NM_000172 |
| 基因名称 | GNAT1 |
| Gene ID (NCBI) | 2779 |
| RRID | AB_3672856 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P11488 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GNAT1, also named as GNATR, belongs to the G-alpha family and G(i/o/t/z) subfamily. Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Transducin is an amplifier and one of the transducers of a visual impulse that performs the coupling between rhodopsin and cGMP-phosphodiesterase. Defects in GNAT1 are the cause of congenital stationary night blindness autosomal dominant type 3 (CSNBAD3). This antibody is specific to GNAT1.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 GNAT1 antibody CL488-55167 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |