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GPT/ALT1 Monoclonal antibody, PBS Only

GPT/ALT1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig, rat, mouse

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5F6B5

Cat no : 67531-1-PBS

Print datasheet

Synonyms

AAT1, Alanine aminotransferase 1, ALAT1, ALT1, GPT, GPT 1, GPT/ALT1, GPT1

验证数据展示

  • IF Staining of HepG2 using 67531-1-Ig (same clone as 67531-1-PBS)

    IF Staining of HepG2 using 67531-1-Ig (same clone as 67531-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GPT antibody (67531-1-Ig, Clone: 5F6B5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67531-1-PBS in a different storage buffer formulation.

  • WB analysis using 67531-1-Ig (same clone as 67531-1-PBS)

    WB analysis using 67531-1-Ig (same clone as 67531-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67531-1-Ig (GPT/ALT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67531-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse liver using 67531-1-Ig (same clone as 67531-1-PBS)

    WB analysis of mouse liver using 67531-1-Ig (same clone as 67531-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 67531-1-Ig (GPT/ALT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67531-1-PBS in a different storage buffer formulation.

产品信息

67531-1-PBS targets GPT/ALT1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Pig, rat, mouse samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, Pig, rat, mouse
Immunogen GPT/ALT1 fusion protein Ag10453 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name glutamic-pyruvate transaminase (alanine aminotransferase)
Synonyms AAT1, Alanine aminotransferase 1, ALAT1, ALT1, GPT, GPT 1, GPT/ALT1, GPT1
Calculated Molecular Weight 496 aa, 55 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC018207
Gene Symbol ALT1
Gene ID (NCBI) 2875
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP24298
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GPT, also known as ALT1 (glutamate-pyruvate transaminase 1), catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.