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GPT/ALT1 Monoclonal antibody

GPT/ALT1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig, rat, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

5F6B5

Cat no : 67531-1-Ig

Print datasheet

Synonyms

AAT1, Alanine aminotransferase 1, ALAT1, ALT1, GPT, GPT 1, GPT/ALT1, GPT1

验证数据展示

  • IF Staining of HepG2 using 67531-1-Ig

    IF Staining of HepG2 using 67531-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GPT antibody (67531-1-Ig, Clone: 5F6B5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • WB analysis using 67531-1-Ig

    WB analysis using 67531-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67531-1-Ig (GPT/ALT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse liver using 67531-1-Ig

    WB analysis of mouse liver using 67531-1-Ig

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 67531-1-Ig (GPT/ALT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inhuman milk, pig heart tissue, pig liver tissue, rat liver tissue, mouse liver tissue, rabbit heart tissue
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67531-1-Ig targets GPT/ALT1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Pig, rat, mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human, Pig, rat, mouse
Immunogen GPT/ALT1 fusion protein Ag10453 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name glutamic-pyruvate transaminase (alanine aminotransferase)
Synonyms AAT1, Alanine aminotransferase 1, ALAT1, ALT1, GPT, GPT 1, GPT/ALT1, GPT1
Calculated Molecular Weight 496 aa, 55 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC018207
Gene Symbol ALT1
Gene ID (NCBI) 2875
RRIDAB_2882750
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP24298
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPT, also known as ALT1 (glutamate-pyruvate transaminase 1), catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.

实验方案

Product Specific Protocols
WB protocol for GPT/ALT1 antibody 67531-1-IgDownload protocol
IF protocol for GPT/ALT1 antibody 67531-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols