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验证数据展示

  • IF Staining of HepG2 using 67531-1-Ig

    IF Staining of HepG2 using 67531-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GPT antibody (67531-1-Ig, Clone: 5F6B5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • WB analysis using 67531-1-Ig

    WB analysis using 67531-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67531-1-Ig (GPT/ALT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse liver using 67531-1-Ig

    WB analysis of mouse liver using 67531-1-Ig

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 67531-1-Ig (GPT/ALT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

GPT/ALT1 Monoclonal antibody

GPT/ALT1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67531-1-Ig

产品说明书

CloneNo. 5F6B5

宿主/亚型

Mouse / IgG1

种属反应性

Human, Pig, rat, mouse

应用

WB, IF/ICC, ELISA

别名

AAT1, Alanine aminotransferase 1, ALAT1, ALT1, GPT, GPT 1, GPT/ALT1, GPT1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman milk, pig heart tissue, pig liver tissue, rat liver tissue, mouse liver tissue, rabbit heart tissue
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67531-1-Ig targets GPT/ALT1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Pig, rat, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, Pig, rat, mouse
免疫原 GPT/ALT1 fusion protein Ag10453 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 glutamic-pyruvate transaminase (alanine aminotransferase)
别名 AAT1, Alanine aminotransferase 1, ALAT1, ALT1, GPT, GPT 1, GPT/ALT1, GPT1
计算分子量 496 aa, 55 kDa
观测分子量 52 kDa
GenBank蛋白编号BC018207
基因名称 ALT1
Gene ID (NCBI) 2875
RRIDAB_2882750
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP24298
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPT, also known as ALT1 (glutamate-pyruvate transaminase 1), catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.

实验方案

Product Specific Protocols
WB protocol for GPT/ALT1 antibody 67531-1-IgDownload protocol
IF protocol for GPT/ALT1 antibody 67531-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols