验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
For other applications, we recommend the unconjugated version of this antibody, 67531-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67531 targets GPT/ALT1 in IF/ICC applications and shows reactivity with Human, Pig samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | Human, Pig |
| 免疫原 | GPT/ALT1 fusion protein Ag10453 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | glutamic-pyruvate transaminase (alanine aminotransferase) |
| 别名 | AAT1, Alanine aminotransferase 1, ALT1, GPT, GPT 1, GPT1 |
| 计算分子量 | 496 aa, 55 kDa |
| 观测分子量 | 52 kDa |
| GenBank蛋白编号 | BC018207 |
| 基因名称 | ALT1 |
| Gene ID (NCBI) | 2875 |
| RRID | AB_2934548 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P24298 |
| 储存缓冲液 | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GPT, also known as ALT1 (glutamate-pyruvate transaminase 1), catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 GPT/ALT1 antibody CL488-67531 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |