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验证数据展示

  • IF Staining of HEK-293 using CL594-67763

    IF Staining of HEK-293 using CL594-67763

    Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using CoraLite®594 GPX4 antibody (CL594-67763, Clone: 3F5G5 ) at dilution of 1:200.

  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated GPX4 Monoclonal antibody

GPX4 Monoclonal Antibody for IF/ICC
Cat No. CL594-67763

Print datasheet

CloneNo. 3F5G5

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat, rabbit, pig

Applications

IF/ICC

Synonyms

Glutathione peroxidase 4, GPx 4, GPX4, GSHPx 4, MCSP, PHGPx, snGPx, snPHGPx

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHEK-293 cells
For other applications, we recommend the unconjugated version of this antibody, 67763-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67763 targets GPX4 in IF/ICC applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, mouse, rat, rabbit, pig
Immunogen GPX4 fusion protein Ag30650 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name glutathione peroxidase 4 (phospholipid hydroperoxidase)
Synonyms Glutathione peroxidase 4, GPx 4, GPX4, GSHPx 4, MCSP, PHGPx, snGPx, snPHGPx
Observed Molecular Weight 20-23 kDa
GenBank Accession NumberBC021567
Gene Symbol GPX4
Gene ID (NCBI) 2879
RRIDAB_2920180
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP36969
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPX4 (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial) protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility.It has two isforms about 20KDa and 22KDa, respectively. GPX4 is a monomer,but it has a tendency to form higher mass oligomers (PMID:17630701). It presents primarily in testis.

实验方案

Product Specific Protocols
IF protocol for CL594 GPX4 antibody CL594-67763Download protocol
Standard Protocols
Click here to view our Standard Protocols