验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HEK-293 cells |
For other applications, we recommend the unconjugated version of this antibody, 67763-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
CL488-67763 targets GPX4 in IF/ICC applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.
| Tested Applications | IF/ICC Application Description |
| Cited Applications | IF |
| Tested Reactivity | Human, mouse, rat, rabbit, pig |
| Cited Reactivity | mouse |
| Immunogen | GPX4 fusion protein Ag30650 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | glutathione peroxidase 4 (phospholipid hydroperoxidase) |
| Synonyms | Glutathione peroxidase 4, GPx 4, GPX4, GSHPx 4, MCSP, PHGPx, snGPx, snPHGPx |
| Observed Molecular Weight | 20-23 kDa |
| GenBank Accession Number | BC021567 |
| Gene Symbol | GPX4 |
| Gene ID (NCBI) | 2879 |
| RRID | AB_2919543 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P36969 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GPX4 (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial) protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility.It has two isforms about 20KDa and 22KDa, respectively. GPX4 is a monomer,but it has a tendency to form higher mass oligomers (PMID:17630701). It presents primarily in testis.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 GPX4 antibody CL488-67763 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |