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验证数据展示

  • IF Staining of mouse kidney using CL488-13892

    IF Staining of mouse kidney using CL488-13892

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using CoraLite® Plus 488 GREM2 antibody (CL488-13892) at dilution of 1:200.

  • FC experiment of HCT 116 using CL488-13892

    FC experiment of HCT 116 using CL488-13892

    1X10^6 HCT 116 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human GREM2 (CL488-13892) (red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated GREM2 Polyclonal antibody

GREM2 Polyclonal Antibody for IF-P, FC (Intra)
Cat No. CL488-13892

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF-P, FC (Intra)

别名

Gremlin2, Gremlin 2, GREM 2, DAND3, DAND 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse kidney tissue
Positive FC (Intra) detected inHCT 116 cells
For other applications, we recommend the unconjugated version of this antibody, 13892-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-13892 targets GREM2 in IF-P, FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 IF-P, FC (Intra) Application Description
经测试反应性 human, mouse
免疫原 GREM2 fusion protein Ag4854 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 gremlin 2, cysteine knot superfamily, homolog (Xenopus laevis)
别名 Gremlin2, Gremlin 2, GREM 2, DAND3, DAND 3
计算分子量 168 aa, 19 kDa
GenBank蛋白编号BC046632
基因名称 GREM2
Gene ID (NCBI) 64388
RRIDAB_3083948
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H772
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 GREM2 antibody CL488-13892Download protocol
FC protocol for CL Plus 488 GREM2 antibody CL488-13892Download protocol
Standard Protocols
Click here to view our Standard Protocols