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验证数据展示

  • IF Staining of HepG2 using CL488-14887

    IF Staining of HepG2 using CL488-14887

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 GRP75 antibody (CL488-14887) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-14887

    FC experiment of HepG2 using CL488-14887

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human GRP75 (CL488-14887) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated GRP75 Polyclonal antibody

GRP75 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-14887

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

HSPA9, Heat shock protein family A member 9, Heat shock 70 kDa protein 9, GRP-75, CSA

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy HSPA9/GRP75 Ready-To-Use IHC Kit. HSPA9/GRP75 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 14887-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-14887 targets GRP75 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 GRP75 fusion protein Ag6673 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 heat shock 70kDa protein 9 (mortalin)
别名 HSPA9, Heat shock protein family A member 9, Heat shock 70 kDa protein 9, GRP-75, CSA
计算分子量 74 kDa
观测分子量 74-75 kDa
GenBank蛋白编号BC000478
基因名称 GRP75
Gene ID (NCBI) 3313
RRIDAB_2919104
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP38646
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GRP75 (also known as mortalin, HSPA9 or mt-Hsp70) is a constitutively expressed member of the HSPA (HSP70) family of heat-shock proteins. It is located in the mitochondrial matrix and anti-GRP75 is commonly used as the marker for mitochondria. It has been reported that GRP75 is enriched in cancer cells and contributes to carcinogenesis. In addition, decreased expression level of GRP75 has been found in neurodegenerative disorders like Parkinson's disease. This antibody recognized the endogenous GRP75 protein in NIH/3T3 cells. (PMID: 21640711, 22920904)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 GRP75 antibody CL488-14887Download protocol
FC protocol for CL Plus 488 GRP75 antibody CL488-14887Download protocol
Standard Protocols
Click here to view our Standard Protocols