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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated GRP75 Polyclonal antibody

GRP75 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-14887

Print datasheet

Synonyms

HSPA9, Heat shock protein family A member 9, Heat shock 70 kDa protein 9, GRP-75, CSA

验证数据展示

  • IF Staining of HepG2 using CL647-14887

    IF Staining of HepG2 using CL647-14887

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 647 GRP75 antibody (CL647-14887) at dilution of 1:200.

  • FC experiment of HepG2 using CL647-14887

    FC experiment of HepG2 using CL647-14887

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human GRP75 (CL647-14887) (red), or 0.2 ug isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy HSPA9/GRP75 Ready-To-Use IHC Kit. HSPA9/GRP75 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 14887-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-14887 targets GRP75 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen GRP75 fusion protein Ag6673 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name heat shock 70kDa protein 9 (mortalin)
Synonyms HSPA9, Heat shock protein family A member 9, Heat shock 70 kDa protein 9, GRP-75, CSA
Calculated Molecular Weight 74 kDa
Observed Molecular Weight 74-75 kDa
GenBank Accession NumberBC000478
Gene Symbol GRP75
Gene ID (NCBI) 3313
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP38646
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GRP75 (also known as mortalin, HSPA9 or mt-Hsp70) is a constitutively expressed member of the HSPA (HSP70) family of heat-shock proteins. It is located in the mitochondrial matrix and anti-GRP75 is commonly used as the marker for mitochondria. It has been reported that GRP75 is enriched in cancer cells and contributes to carcinogenesis. In addition, decreased expression level of GRP75 has been found in neurodegenerative disorders like Parkinson's disease. This antibody recognized the endogenous GRP75 protein in NIH/3T3 cells. (PMID: 21640711, 22920904)

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 GRP75 antibody CL647-14887Download protocol
FC protocol for CL Plus 647 GRP75 antibody CL647-14887Download protocol
Standard Protocols
Click here to view our Standard Protocols