验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, A549 cells, Jurkat cells |
Positive FC (Intra) detected in | RAW 264.7 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:2000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
80918-1-RR targets GSDMD in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, FC (Intra), ELISA Application Description |
经测试反应性 | human, mouse |
免疫原 | GSDMD fusion protein Ag14757 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | gasdermin D |
别名 | Gasdermin-D, gasderminD, Gasdermin domain-containing protein 1, gasdermin D, FKSG10 |
计算分子量 | 484 aa, 53 kDa |
观测分子量 | 50-53 kDa |
GenBank蛋白编号 | BC008904 |
基因名称 | GSDMD |
Gene ID (NCBI) | 79792 |
RRID | AB_2918919 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P57764 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GSDMD is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. GSDMD has been suggested to act as a tumor suppressor. Recently, GSDMD is identified as a new component of inflammasomes, and it is an executor of pyroptosis and required for interleukin-1β secretion. Cleavage of GSDMD by inflammatory caspases determines pyroptotic cell death.
实验方案
Product Specific Protocols | |
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WB protocol for GSDMD antibody 80918-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |