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CoraLite® Plus 488-conjugated GSDMD Recombinant antibody

GSDMD Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2N24

Cat No : CL488-80918

Print datasheet

Synonyms

DF5L, DFNA5L, FKSG10, FLJ12150, gasdermin D, GSDMD, GSDMDC1

验证数据展示

  • FC experiment of RAW 264.7 using CL488-80918

    FC experiment of RAW 264.7 using CL488-80918

    1X10^6 RAW 264.7 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human GSDMD (CL488-80918, Clone:2N24) (red), or 0.8 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inRAW 264.7 cells
For other applications, we recommend the unconjugated version of this antibody, 80918-1-PBS

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-80918 targets GSDMD in FC (Intra) applications and shows reactivity with Human, mouse samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity Human, mouse
Immunogen GSDMD fusion protein Ag14757 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name gasdermin D
Synonyms DF5L, DFNA5L, FKSG10, FLJ12150, gasdermin D, GSDMD, GSDMDC1
Calculated Molecular Weight 484 aa, 53 kDa
Observed Molecular Weight50-53 kDa
GenBank Accession NumberBC008904
Gene Symbol GSDMD
Gene ID (NCBI) 79792
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP57764
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GSDMD is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. GSDMD has been suggested to act as a tumor suppressor. Recently, GSDMD is identified as a new component of inflammasomes, and it is an executor of pyroptosis and required for interleukin-1β secretion. Cleavage of GSDMD by inflammatory caspases determines pyroptotic cell death.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 GSDMD antibody CL488-80918Download protocol
Standard Protocols
Click here to view our Standard Protocols