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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated GSNOR,ADH5 Polyclonal antibody

GSNOR,ADH5 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-16379

Print datasheet

Synonyms

ADH5, Alcohol dehydrogenase class-3, ADHX, ADH3, ADH 5

验证数据展示

  • FC experiment of HepG2 using CL488-16379

    FC experiment of HepG2 using CL488-16379

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated GSNOR,ADH5 Polyclonal antibody (CL488-16379)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 16379-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-16379 targets GSNOR,ADH5 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen GSNOR,ADH5 fusion protein Ag9360 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name alcohol dehydrogenase 5 (class III), chi polypeptide
Synonyms ADH5, Alcohol dehydrogenase class-3, ADHX, ADH3, ADH 5
Calculated Molecular Weight 374 aa, 40 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC014665
Gene Symbol ADH5
Gene ID (NCBI) 128
RRIDAB_2919133
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP11766
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ADH5, also named as ADHX, FDH FALDH GSH-FDH ADH3 and GSNOR, belongs to the zinc-containing alcohol dehydrogenase family and Class-III subfamily. It is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione. ADH5 mediates multiple cardiovascular functions. It plays in regulating heterocellular communication in the artery wall. (PMID:21071693). ADH5 immunostaining is distributed in both the nucleus and cytoplasm of the retinal ganglion cells(PMID:22117533).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 GSNOR,ADH5 antibody CL488-16379Download protocol
Standard Protocols
Click here to view our Standard Protocols