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验证数据展示

  • WB analysis using 13897-1-AP (same clone as 13897-1-PBS)

    WB analysis using 13897-1-AP (same clone as 13897-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13897-1-AP (GTPBP4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13897-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 13897-1-AP (same clone as 13897-1-PBS)

    IP experiment of HeLa using 13897-1-AP (same clone as 13897-1-PBS)

    IP result of anti-GTPBP4 (IP:13897-1-AP, 4ug; Detection:13897-1-AP 1:5000) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 13897-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse testis using 13897-1-AP (same clone as 13897-1-PBS)

    IP experiment of mouse testis using 13897-1-AP (same clone as 13897-1-PBS)

    IP result of anti-GTPBP4 (IP:13897-1-AP, 4ug; Detection:13897-1-AP 1:800) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 13897-1-PBS in a different storage buffer formulation.

  • IF Staining of MDCK using 13897-1-AP (same clone as 13897-1-PBS)

    IF Staining of MDCK using 13897-1-AP (same clone as 13897-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MDCK cells using GTPBP4 antibody (13897-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 13897-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

GTPBP4 Polyclonal antibody, PBS Only

GTPBP4 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 13897-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, IP, Indirect ELISA

别名

NGB, GTP-binding protein NGB, GTP-binding protein 4, GTP binding protein NGB, GTP binding protein 4

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

13897-1-PBS targets GTPBP4 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 GTPBP4 fusion protein Ag4859 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 GTP binding protein 4
别名 NGB, GTP-binding protein NGB, GTP-binding protein 4, GTP binding protein NGB, GTP binding protein 4
计算分子量 634 aa, 74 kDa
观测分子量 74 kDa
GenBank蛋白编号BC038975
基因名称 GTPBP4
Gene ID (NCBI) 23560
RRIDAB_2279486
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BZE4
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GTP-binding proteins are GTPases and function as molecular switches that can flip between two states: active, when GTP is bound, and inactive, when GDP is bound. 'Active' in this context usually means that the molecule acts as a signal to trigger other events in the cell. When an extracellular ligand binds to a G-protein-linked receptor, the receptor changes its conformation and switches on the trimeric G proteins that associate with it by causing them to eject their GDP and replace it with GTP. The switch is turned off when the G protein hydrolyzes its own bound GTP, converting it back to GDP. But before that occurs, the active protein has an opportunity to diffuse away from the receptor and deliver its message for a prolonged period to its downstream target.