CoraLite® Plus 488-conjugated Geminin Polyclonal antibody
Geminin Polyclonal Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
验证数据展示
经过测试的应用
Positive IF/ICC detected in | HepG2 cells |
For other applications, we recommend the unconjugated version of this antibody, 10802-1-AP
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-10802 targets Geminin in IF/ICC applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF/ICC Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | Geminin fusion protein Ag1076 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | geminin, DNA replication inhibitor |
Synonyms | Gem, Geminin, GMNN, RP3 369A17.3 |
Calculated Molecular Weight | 24 kDa |
Observed Molecular Weight | 28-29 kDa |
GenBank Accession Number | BC005185 |
Gene Symbol | GMNN |
Gene ID (NCBI) | 51053 |
RRID | AB_2918997 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O75496 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GMNN, also designated geminin, contains 212 amino acids and has a destruction box sequence (RRTLKVIQP). GMNN participates in ininhibiting DNA replication by preventing the incorporation of MCM complex into pre-replication complex (pre-RC). It is degraded during the metaphase-anaphase transition of cell cycle's mitotic phase, which permits replication in the succeeding cell cycle. GMNN has a broad sedimentation profile ranging from about 25 kDa to 90 kDa, with a major peak at 30 kDa. Scanning of the signals shows that discrete peaks corresponding to apparent mass of 42.5 kDa and 66 kDa are present. (PMID: 15313623)
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 Geminin antibody CL488-10802 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |