CoraLite®555-conjugated Geminin Polyclonal antibody
Geminin Polyclonal Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC
Conjugate
CoraLite®555 Fluorescent Dye
验证数据展示
经过测试的应用
| Positive IF detected in | HepG2 cells |
For other applications, we recommend the unconjugated version of this antibody, 10802-1-AP
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
CL555-10802 targets Geminin in IF applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC Application Description |
| Cited Applications | IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Immunogen | Geminin fusion protein Ag1076 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | geminin, DNA replication inhibitor |
| Synonyms | Gem, Geminin, GMNN, RP3 369A17.3 |
| Calculated Molecular Weight | 24 kDa |
| Observed Molecular Weight | 28-29 kDa |
| GenBank Accession Number | BC005185 |
| Gene Symbol | GMNN |
| Gene ID (NCBI) | 51053 |
| RRID | AB_2919592 |
| Conjugate | CoraLite®555 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 557 nm / 570nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75496 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GMNN, also designated geminin, contains 212 amino acids and has a destruction box sequence (RRTLKVIQP). GMNN participates in ininhibiting DNA replication by preventing the incorporation of MCM complex into pre-replication complex (pre-RC). It is degraded during the metaphase-anaphase transition of cell cycle's mitotic phase, which permits replication in the succeeding cell cycle. GMNN has a broad sedimentation profile ranging from about 25 kDa to 90 kDa, with a major peak at 30 kDa. Scanning of the signals shows that discrete peaks corresponding to apparent mass of 42.5 kDa and 66 kDa are present. (PMID: 15313623)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL555 Geminin antibody CL555-10802 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Rep RAD52-dependent mitotic DNA synthesis is required for genome stability in Cyclin E1-overexpressing cells |