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验证数据展示

  • FC experiment of HeLa using CL488-12645

    FC experiment of HeLa using CL488-12645

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Glnrs Polyclonal Antibody (CL488-12645)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of PC-3 using CL488-12645

    IF Staining of PC-3 using CL488-12645

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using CoraLite® Plus 488 GlnRS antibody (CL488-12645) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated GlnRS Polyclonal antibody

GlnRS Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-12645

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

QARS, SYQ, QARS1, Glutaminyl-tRNA synthetase, Glutamine--tRNA ligase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inPC-3 cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 12645-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-12645 targets GlnRS in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 GlnRS fusion protein Ag3375 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glutaminyl-tRNA synthetase
别名 QARS, SYQ, QARS1, Glutaminyl-tRNA synthetase, Glutamine--tRNA ligase
计算分子量 775 aa, 88 kDa
观测分子量 88 kDa
GenBank蛋白编号BC016634
基因名称 GlnRS
Gene ID (NCBI) 5859
RRIDAB_3672545
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP47897
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GLnRS(glutaminyl-tRNA synthetase) is a class I aminoacyl-tRNA synthetase. Aminoacyl-tRNA synthetases are enzymes that charge tRNAs with their cognate amino acids. The specificity of this reaction determines the fidelity of mRNA translation. At least 1 synthetase exists in the cytoplasm for each amino acid.GlnRS is present in eukaryotes but is absent from many prokaryotes, mitochondria, and chloroplasts, in which Gln-tRNA(Gln) is formed by transamidation of the misacylated Glu-tRNA(Gln)(PMID:8078941).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 GlnRS antibody CL488-12645Download protocol
FC protocol for CL Plus 488 GlnRS antibody CL488-12645Download protocol
Standard Protocols
Click here to view our Standard Protocols