验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy NR3C1 Ready-To-Use IHC Kit. NR3C1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 82619-4-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-82619-4 targets Glucocorticoid receptor in IF/ICC applications and shows reactivity with Human, Mouse samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | Human, Mouse |
| 免疫原 | Glucocorticoid receptor fusion protein Ag21146 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) |
| 别名 | GCCR, GCR, Glucocorticoid receptor, GR, GRL, NR3C1 |
| 计算分子量 | 86 kDa |
| 观测分子量 | 94-97 kDa |
| GenBank蛋白编号 | BC015610 |
| 基因名称 | Glucocorticoid receptor |
| Gene ID (NCBI) | 2908 |
| RRID | AB_3084516 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P04150 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Glucocorticoid receptor (GR, or GCR) also known as NR3C1 (nuclear receptor subfamily 3, group C, member 1) is a receptor for glucocorticoids, which owns a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. It is involved in cell proliferation and differentiation and specifically implicated in newborn birth weight, thus providing a biological mechanism by which NR3C1 expression may influence birth weight (PMID:22810058).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Glucocorticoid receptor antibody CL488-82619-4 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
