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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 83859-1-RR (same clone as 83859-1-PBS)

    WB analysis using 83859-1-RR (same clone as 83859-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83859-1-RR (HEC1/HEC/NDC80 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83859-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83859-1-RR (same clone as 83859-1-PBS)

    FC experiment of HeLa using 83859-1-RR (same clone as 83859-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug HEC1/HEC/NDC80 Recombinant antibody (83859-1-RR, Clone:5A2) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83859-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83859-1-RR (same clone as 83859-1-PBS)

    IF Staining of HeLa using 83859-1-RR (same clone as 83859-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HEC1/HEC/NDC80 antibody (83859-1-RR, Clone: 5A2 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 83859-1-PBS in a different storage buffer formulation.

HEC1/HEC/NDC80 Recombinant antibody, PBS Only

HEC1/HEC/NDC80 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83859-1-PBS
CloneNo. 5A2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

NDC80, Kinetochore protein Hec1, hsNDC80, HsHec1, HEC1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

83859-1-PBS targets HEC1/HEC/NDC80 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name NDC80 homolog, kinetochore complex component (S. cerevisiae)
Synonyms NDC80, Kinetochore protein Hec1, hsNDC80, HsHec1, HEC1
Observed Molecular Weight 74 kDa
GenBank Accession Number/
Gene Symbol NDC80
Gene ID (NCBI) 10403
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NDC80 complex is a heterotetrameric protein complex comprised of Hec1 (also called Ndc80), Nuf2, Spc24, and Spc25. Mis12 complex (bound to CENP-C or CENP-T) or CDK1-phosphorylated CENP-T to anchor the NDC80 complex to the kinetochore. HEC is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved.