CoraLite® Plus 488-conjugated HEC1/HEC/NDC80 Recombinant antibody
HEC1/HEC/NDC80 Uni-rAbTM Recombinant Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
5A2
验证数据展示
经过测试的应用
Positive IF/ICC detected in | HeLa cells |
For other applications, we recommend the unconjugated version of this antibody, 83859-1-PBS
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-83859 targets HEC1/HEC/NDC80 in IF/ICC applications and shows reactivity with human samples.
Tested Applications | IF/ICC Application Description |
Tested Reactivity | human |
Immunogen | Fusion Protein 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | NDC80 homolog, kinetochore complex component (S. cerevisiae) |
Synonyms | NDC80, Kinetochore protein Hec1, hsNDC80, HsHec1, HEC1 |
Observed Molecular Weight | 74 kDa |
GenBank Accession Number | / |
Gene Symbol | NDC80 |
Gene ID (NCBI) | 10403 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NDC80 complex is a heterotetrameric protein complex comprised of Hec1 (also called Ndc80), Nuf2, Spc24, and Spc25. Mis12 complex (bound to CENP-C or CENP-T) or CDK1-phosphorylated CENP-T to anchor the NDC80 complex to the kinetochore. HEC is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved.
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 HEC1/HEC/NDC80 antibody CL488-83859 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |