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HIBCH Recombinant antibody

HIBCH Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240590A3

Cat no : 83569-1-RR

Print datasheet

Synonyms

HIB-CoA hydrolase, HIB CoA hydrolase, EC:3.1.2.4, 3-hydroxyisobutyryl-coenzyme A hydrolase, 3-hydroxyisobutyryl-CoA hydrolase, mitochondrial

验证数据展示

  • WB analysis using 83569-1-RR

    WB analysis using 83569-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83569-1-RR (HIBCH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 83569-1-RR

    IF Staining of HepG2 using 83569-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HIBCH antibody (83569-1-RR, Clone: 240590A3 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • FC experiment of HepG2 using 83569-1-RR

    FC experiment of HepG2 using 83569-1-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug HIBCH Recombinant antibody (83569-1-RR, Clone:240590A3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83569-1-RR

    Affinity and Kinetic Characterization of 83569-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83569-1-RR against Human HIBCH were performed. The affinity constant is 1.37 nM.

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, rat liver tissue
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy HIBCH Ready-To-Use IHC Kit. HIBCH primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83569-1-RR is HIBCH Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, rat
Immunogen HIBCH fusion protein Ag6149 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name 3-hydroxyisobutyryl-Coenzyme A hydrolase
Synonyms HIB-CoA hydrolase, HIB CoA hydrolase, EC:3.1.2.4, 3-hydroxyisobutyryl-coenzyme A hydrolase, 3-hydroxyisobutyryl-CoA hydrolase, mitochondrial
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC067822
Gene Symbol HIBCH
Gene ID (NCBI) 26275
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ6NVY1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HIBCH belongs to the enoyl-CoA hydratase/isomerase family. HIBCH has two isoforms with MW 43 kDa and 38 kDa. It is highly expressed in the liver and kidney, also detected in the heart, muscle and brain (at protein level), but not detected in the lung. Hydrolyzes 3-hydroxyisobutyryl-CoA (HIBYL-CoA), a saline catabolite. Has high activity toward isobutyryl-CoA. Could be an isobutyryl-CoA dehydrogenase that functions in valine catabolism. Also hydrolyzes 3-hydroxypropanoyl-CoA (PMID: 8824301).

实验方案

Product Specific Protocols
WB protocol for HIBCH antibody 83569-1-RRDownload protocol
IF protocol for HIBCH antibody 83569-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols