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验证数据展示

  • WB analysis of Daudi using 82922-1-RR (same clone as 82922-1-PBS)

    WB analysis of Daudi using 82922-1-RR (same clone as 82922-1-PBS)

    WB result of HLA-DMB antibody (82922-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HLA-DMB transfected Daudi cells. This data was developed using the same antibody clone with 82922-1-PBS in a different storage buffer formulation.

  • WB analysis using 82922-1-RR (same clone as 82922-1-PBS)

    WB analysis using 82922-1-RR (same clone as 82922-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82922-1-RR (HLA-DMB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82922-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 82922-1-RR (same clone as 82922-1-PBS)

    IHC staining of human ovary cancer using 82922-1-RR (same clone as 82922-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 82922-1-RR (HLA-DMB antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82922-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 82922-1-RR (same clone as 82922-1-PBS)

    IHC staining of human ovary cancer using 82922-1-RR (same clone as 82922-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 82922-1-RR (HLA-DMB antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82922-1-PBS in a different storage buffer formulation.

  • IF Staining of Raji using 82922-1-RR (same clone as 82922-1-PBS)

    IF Staining of Raji using 82922-1-RR (same clone as 82922-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using HLA-DMB antibody (82922-1-RR, Clone: 230123A3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82922-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82922-1-RR

    Affinity and Kinetic Characterization of 82922-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82922-1-RR against Human HLA-DMB were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

HLA-DMB Recombinant antibody, PBS Only

HLA-DMB Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 82922-1-PBS

产品说明书

CloneNo. 230123A3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

RING7, MHC class II antigen DMB, HLA DMB, DMB, 230123A3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

82922-1-PBS targets HLA-DMB in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 HLA-DMB fusion protein Ag16320 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 major histocompatibility complex, class II, DM beta
别名 RING7, MHC class II antigen DMB, HLA DMB, DMB, 230123A3
计算分子量 263 aa, 29 kDa
观测分子量31 kDa
GenBank蛋白编号BC027175
基因名称 HLA-DMB
Gene ID (NCBI) 3109
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP28068
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). The class II molecules are composed of two non-covalently associated alpha and beta chains. HLA-DMA and HLA-DMB form a functional heterodimer that is critical in the pathway of class II antigen presentation. HLA-DM plays a critical role in catalyzing the release of Class II HLA-associated invariant chain-derived peptides (CLIP) from newly synthesized class II HLA molecules and freeing the peptide binding site for acquisition of antigenic peptides.