HLA-DMB Recombinant antibody
HLA-DMB Recombinant Antibody for WB, FC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
WB, FC, ELISA
Conjugate
Unconjugated
CloneNo.
230123A3
验证数据展示
经过测试的应用
| Positive WB detected in | Raji cells, Daudi cells, Ramos cells |
| Positive FC detected in | Raji cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
82922-1-RR targets HLA-DMB in WB, FC, ELISA applications and shows reactivity with Human samples.
| Tested Applications | WB, FC, ELISA Application Description |
| Tested Reactivity | Human |
| Immunogen | HLA-DMB fusion protein Ag16320 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | major histocompatibility complex, class II, DM beta |
| Synonyms | D6S221E, DMB, HLA DMB, HLA-DMB, MHC class II antigen DMB, RING7 |
| Calculated Molecular Weight | 263 aa, 29 kDa |
| Observed Molecular Weight | 31 kDa |
| GenBank Accession Number | BC027175 |
| Gene Symbol | HLA-DMB |
| Gene ID (NCBI) | 3109 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P28068 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). The class II molecules are composed of two non-covalently associated alpha and beta chains. HLA-DMA and HLA-DMB form a functional heterodimer that is critical in the pathway of class II antigen presentation. HLA-DM plays a critical role in catalyzing the release of Class II HLA-associated invariant chain-derived peptides (CLIP) from newly synthesized class II HLA molecules and freeing the peptide binding site for acquisition of antigenic peptides.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HLA-DMB antibody 82922-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
