验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, K-562 cells, C6 cells, T-47D cells |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15820-1-AP targets HMMR-Specific in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | hyaluronan-mediated motility receptor (RHAMM) |
| 别名 | HMMR, CD168, RHAMM, Receptor for hyaluronan-mediated motility, Intracellular hyaluronic acid-binding protein |
| 计算分子量 | 84 kDa |
| 观测分子量 | 84 kDa |
| GenBank蛋白编号 | NM_001142556 |
| 基因名称 | HMMR |
| Gene ID (NCBI) | 3161 |
| RRID | AB_2295342 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O75330 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Hyaluronan-mediated motility receptor (HMMR), also termed CD168, was first described by Turley in murine cells . It is reported that HMMR has an extensive coiled-coil structure (CC) that contains multiple sites for interactive partners. Initially, HMMR was considered a novel hyaluronan-mediated motility receptor and a microtubule-associated spindle assembly factor. Full-length human RHAMM is an 85 kDa coiled-coil protein that occurs both in intracellular and extracellular compartments. It has highly restricted and tightly regulated expression in most normal tissues, but is one of a number of oncogenic proteins that are exported to the cell surface in response to tissue stress by unconventional transport mechanisms. (PMID: 36750558, PMID: 30249497)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HMMR-Specific antibody 15820-1-AP | Download protocol |
| IHC protocol for HMMR-Specific antibody 15820-1-AP | Download protocol |
| IF protocol for HMMR-Specific antibody 15820-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Transl Med Integrative multi-omics analysis unveils stemness-associated molecular subtypes in prostate cancer and pan-cancer: prognostic and therapeutic significance | ||
Int J Mol Sci Effects of Therapeutic Platelet-Rich Plasma on Overactive Bladder via Modulating Hyaluronan Synthesis in Ovariectomized Rat | ||
Int J Biol Sci Oleate acid-stimulated HMMR expression by CEBPα is associated with nonalcoholic steatohepatitis and hepatocellular carcinoma.
| ||
J Cell Physiol A pH-sensitive luminal His-cluster promotes interaction of PAM with V-ATPase along the secretory and endocytic pathways of peptidergic cells. | ||
Acta Biochim Biophys Sin (Shanghai) FOXM1 mediates methotrexate resistance in osteosarcoma cells by promoting autophagy |