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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated HMMR-Specific Polyclonal antibody

HMMR-Specific Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-15820

Print datasheet

Synonyms

HMMR, CD168, RHAMM, Receptor for hyaluronan-mediated motility, Intracellular hyaluronic acid-binding protein

验证数据展示

  • FC experiment of HepG2 using CL488-15820

    FC experiment of HepG2 using CL488-15820

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human HMMR-Specific (CL488-15820) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 15820-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-15820 targets HMMR-Specific in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name hyaluronan-mediated motility receptor (RHAMM)
Synonyms HMMR, CD168, RHAMM, Receptor for hyaluronan-mediated motility, Intracellular hyaluronic acid-binding protein
Calculated Molecular Weight 84 kDa
GenBank Accession NumberNM_001142556
Gene Symbol HMMR
Gene ID (NCBI) 3161
RRIDAB_2919123
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75330
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Hyaluronan-mediated motility receptor (HMMR), also termed CD168, was first described by Turley in murine cells . It is reported that HMMR has an extensive coiled-coil structure (CC) that contains multiple sites for interactive partners. Initially, HMMR was considered a novel hyaluronan-mediated motility receptor and a microtubule-associated spindle assembly factor. Full-length human RHAMM is an 85  kDa coiled-coil protein that occurs both in intracellular and extracellular compartments. It has highly restricted and tightly regulated expression in most normal tissues, but is one of a number of oncogenic proteins that are exported to the cell surface in response to tissue stress by unconventional transport mechanisms. (PMID: 36750558, PMID: 30249497)

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 HMMR-Specific antibody CL488-15820Download protocol
Standard Protocols
Click here to view our Standard Protocols