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验证数据展示

  • FC experiment of HepG2 using CL555-15820

    FC experiment of HepG2 using CL555-15820

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human HMMR-Specific (CL555-15820) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HepG2 using CL555-15820

    IF Staining of HepG2 using CL555-15820

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®555 HMMR-Specific antibody (CL555-15820) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite®555-conjugated HMMR-Specific Polyclonal antibody

HMMR-Specific Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL555-15820

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

HMMR, CD168, RHAMM, Receptor for hyaluronan-mediated motility, Intracellular hyaluronic acid-binding protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®555
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 15820-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-15820 targets HMMR-Specific in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 hyaluronan-mediated motility receptor (RHAMM)
别名 HMMR, CD168, RHAMM, Receptor for hyaluronan-mediated motility, Intracellular hyaluronic acid-binding protein
计算分子量 84 kDa
观测分子量 70 kDa
GenBank蛋白编号NM_001142556
基因名称 HMMR
Gene ID (NCBI) 3161
RRIDAB_2919628
偶联类型 CoraLite®555 Fluorescent Dye
最大激发/发射波长557 nm / 570 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75330
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Hyaluronan-mediated motility receptor (HMMR), also termed CD168, was first described by Turley in murine cells . It is reported that HMMR has an extensive coiled-coil structure (CC) that contains multiple sites for interactive partners. Initially, HMMR was considered a novel hyaluronan-mediated motility receptor and a microtubule-associated spindle assembly factor. Full-length human RHAMM is an 85  kDa coiled-coil protein that occurs both in intracellular and extracellular compartments. It has highly restricted and tightly regulated expression in most normal tissues, but is one of a number of oncogenic proteins that are exported to the cell surface in response to tissue stress by unconventional transport mechanisms. (PMID: 36750558, PMID: 30249497)

实验方案

Product Specific Protocols
IF protocol for CL555 HMMR-Specific antibody CL555-15820Download protocol
FC protocol for CL555 HMMR-Specific antibody CL555-15820Download protocol
Standard Protocols
Click here to view our Standard Protocols