CoraLite® Plus 488-conjugated HNRNPA1 Monoclonal antibody

HNRNPA1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A5A4

Cat no : CL488-67844

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Synonyms

Helix destabilizing protein, hnRNP A1, hnRNP core protein A1, HNRNPA1, HNRPA1



经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy HNRNPA1 Ready-To-Use IHC Kit. HNRNPA1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67844-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67844 targets HNRNPA1 in IF/ICC applications and shows reactivity with Human, Mouse samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, Mouse
Immunogen HNRNPA1 fusion protein Ag17415 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name heterogeneous nuclear ribonucleoprotein A1
Synonyms Helix destabilizing protein, hnRNP A1, hnRNP core protein A1, HNRNPA1, HNRPA1
Calculated Molecular Weight 34 kDa
Observed Molecular Weight34 kDa
GenBank Accession NumberBC009600
Gene Symbol HNRNPA1
Gene ID (NCBI) 3178
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP09651
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

In eukaryotic cells, nascent RNA polymerase II transcripts are associated in the nucleus with specific proteins to form ribonucleoprotein complexes called HNRP or 40S. Protein moiety of the HNRP particle has 6 major components called core proteins, and HNRPA1 is one of them. hnRNP A1 is a multifunctional RNA binding protein which is involved in pre-mRNA splicing, export of mature transcripts from the nucleus, mRNA turnover, and internal ribosome entry site (IRES)-mediated translation. HNRNPA1 has three isoforms with MW 30 kDa, 34 kDa and 39 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 HNRNPA1 antibody CL488-67844Download protocol
Standard Protocols
Click here to view our Standard Protocols