验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy HNRNPA1 Ready-To-Use IHC Kit. HNRNPA1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67844-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67844 targets HNRNPA1 in IF/ICC applications and shows reactivity with Human, Mouse samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | Human, Mouse |
| 免疫原 | HNRNPA1 fusion protein Ag17415 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | heterogeneous nuclear ribonucleoprotein A1 |
| 别名 | Helix destabilizing protein, hnRNP A1, hnRNP core protein A1, HNRNPA1, HNRPA1 |
| 计算分子量 | 34 kDa |
| 观测分子量 | 34 kDa |
| GenBank蛋白编号 | BC009600 |
| 基因名称 | HNRNPA1 |
| Gene ID (NCBI) | 3178 |
| RRID | AB_2923800 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P09651 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
In eukaryotic cells, nascent RNA polymerase II transcripts are associated in the nucleus with specific proteins to form ribonucleoprotein complexes called HNRP or 40S. Protein moiety of the HNRP particle has 6 major components called core proteins, and HNRPA1 is one of them. hnRNP A1 is a multifunctional RNA binding protein which is involved in pre-mRNA splicing, export of mature transcripts from the nucleus, mRNA turnover, and internal ribosome entry site (IRES)-mediated translation. HNRNPA1 has three isoforms with MW 30 kDa, 34 kDa and 39 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 HNRNPA1 antibody CL488-67844 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |