CoraLite®594-conjugated HNRNPA1 Monoclonal antibody
HNRNPA1 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse
Applications
IF/ICC
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
1A5A4
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy HNRNPA1 Ready-To-Use IHC Kit. HNRNPA1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67844-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-67844 targets HNRNPA1 in IF/ICC applications and shows reactivity with human, mouse samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | human, mouse |
| Immunogen | HNRNPA1 fusion protein Ag17415 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | heterogeneous nuclear ribonucleoprotein A1 |
| Synonyms | hnRNP core protein A1, hnRNP A1, Heterogeneous nuclear ribonucleoprotein A1, N-terminally processed, Helix-destabilizing protein, Helix destabilizing protein |
| Calculated Molecular Weight | 34 kDa |
| Observed Molecular Weight | 34 kDa |
| GenBank Accession Number | BC009600 |
| Gene Symbol | HNRNPA1 |
| Gene ID (NCBI) | 3178 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P09651 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
In eukaryotic cells, nascent RNA polymerase II transcripts are associated in the nucleus with specific proteins to form ribonucleoprotein complexes called HNRP or 40S. Protein moiety of the HNRP particle has 6 major components called core proteins, and HNRPA1 is one of them. hnRNP A1 is a multifunctional RNA binding protein which is involved in pre-mRNA splicing, export of mature transcripts from the nucleus, mRNA turnover, and internal ribosome entry site (IRES)-mediated translation. HNRNPA1 has three isoforms with MW 30 kDa, 34 kDa and 39 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 HNRNPA1 antibody CL594-67844 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |