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CoraLite® Plus 488-conjugated HNRNPD Polyclonal antibody
HNRNPD Polyclonal Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
验证数据展示
经过测试的应用
| Positive IF detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy HNRNPD Ready-To-Use IHC Kit. HNRNPD primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 12770-1-AP
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-12770 targets HNRNPD in IF applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | human, mouse, rat |
| Immunogen | HNRNPD fusion protein Ag3458 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37kDa) |
| Synonyms | AUF1, AUF1A, hnRNP D0, HNRNPD, hnRNPD0, HNRPD, P37 |
| Calculated Molecular Weight | 355 aa, 38 kDa |
| Observed Molecular Weight | 38-45 kDa |
| GenBank Accession Number | BC026015 |
| Gene Symbol | HNRNPD |
| Gene ID (NCBI) | 3184 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q14103 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. HNRNPD, also known as AUF1, belongs to the family of AU-binding proteins (AU-BPs) that regulate the cellular half-lives of many mRNAs by directly interacting with an AU-rich element (ARE) located in their 3′ untranslated region [PMID:8578590,12704645]. AUF1 has four isoforms produced by alternative splicing of a single transcript: p37, p40, p42, and p45 (PMID:9521873).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 HNRNPD antibody CL488-12770 | Download protocol |
| FC protocol for CL Plus 488 HNRNPD antibody CL488-12770 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
