HPSE Recombinant antibody

HPSE Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 84562-1-RR

产品说明书

CloneNo. 241651E3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, FC (Intra), ELISA

Heparanase, HEP, Endo-glucoronidase, Endo glucoronidase, EC:3.2.1.166

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84562-1-RR targets HPSE in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 HPSE fusion protein Ag21479 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 heparanase
别名 Heparanase, HEP, Endo-glucoronidase, Endo glucoronidase, EC:3.2.1.166
计算分子量 543 aa, 61 kDa
观测分子量 50 kDa
GenBank蛋白编号BC051321
基因名称 HPSE
Gene ID (NCBI) 10855
RRIDAB_3672070
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9Y251
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HPSE(Heparanase) is also named as HEP, HPA, HPA1, HPR1, HPSE1, HSE1 and belongs to the glycosyl hydrolase 79 family. It is a endoglycosidase that cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. HPSE is essential in the disassembly of the extracellular matrix (ECM) by invading cells. It has 3 isoforms produced by alternative splicing with the molecular weight of 61 kDa, 55 kDa and 53 kDa. The full length protein has six glycosylation sites. The cleavage of the 65 kDa form leads to the generation of a linker peptide, and 8 kDa and 50 kDa products. The active form, the 8/50 kDa heterodimer, is resistant to degradation and glycosylation of the 50 kDa subunit appears to be essential for its solubility.

实验方案

Product Specific Protocols
WB protocol for HPSE antibody 84562-1-RRDownload protocol
IHC protocol for HPSE antibody 84562-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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