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HSF1 Polyclonal antibody, PBS Only

HSF1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 16107-1-PBS

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Synonyms

验证数据展示

  • WB analysis of K-562 using 16107-1-AP (same clone as 16107-1-PBS)

    WB analysis of K-562 using 16107-1-AP (same clone as 16107-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 16107-1-AP (HSF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16107-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 16107-1-AP (same clone as 16107-1-PBS)

    IHC staining of human lung cancer using 16107-1-AP (same clone as 16107-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16107-1-AP (HSF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16107-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HSF1 antibody included
  • IHC staining of human cervical cancer using 16107-1-AP (same clone as 16107-1-PBS)

    IHC staining of human cervical cancer using 16107-1-AP (same clone as 16107-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16107-1-AP (HSF1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16107-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HSF1 antibody included
Planning an IHC experiment? We recommend our IHCeasy HSF1 Ready-To-Use IHC Kit. HSF1 primary antibody included.

产品信息

16107-1-PBS targets HSF1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen HSF1 fusion protein Ag9023 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name heat shock transcription factor 1
Synonyms
Calculated Molecular Weight 529 aa, 57 kDa
Observed Molecular Weight 68-80 kDa
GenBank Accession NumberBC014638
Gene Symbol HSF1
Gene ID (NCBI) 3297
RRIDAB_11182610
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ00613
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HSF1 belongs to heat-shock transcription factors that activate heat-shock response genes under conditions of heat or other stresses. Also, HSF1 has been linked with oogenesis, spermatogenesis, and placental development. It can activate AKT and inactivate JNK and CASP3 to protect cardiomyocytes from death. And it has a role in the regulation of life span and establishes a role for SIRT1 in protein homeostasis and heat-shock response. The calculated molecular weight of HSF1 is 57 kDa, but HSF1 migrates at approximately 80 kDa which likely represents different phosphorylation states (PMID: 18434628).