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验证数据展示

  • WB analysis of K-562 using 16107-1-AP (same clone as 16107-1-PBS)

    WB analysis of K-562 using 16107-1-AP (same clone as 16107-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 16107-1-AP (HSF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16107-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 16107-1-AP (same clone as 16107-1-PBS)

    IHC staining of human lung cancer using 16107-1-AP (same clone as 16107-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16107-1-AP (HSF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16107-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HSF1 antibody included
  • IHC staining of human cervical cancer using 16107-1-AP (same clone as 16107-1-PBS)

    IHC staining of human cervical cancer using 16107-1-AP (same clone as 16107-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16107-1-AP (HSF1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16107-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HSF1 antibody included

HSF1 Polyclonal antibody, PBS Only

HSF1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 16107-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy HSF1 Ready-To-Use IHC Kit. HSF1 primary antibody included.

产品信息

16107-1-PBS targets HSF1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 HSF1 fusion protein Ag9023 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 heat shock transcription factor 1
别名
计算分子量 529 aa, 57 kDa
观测分子量 68-80 kDa
GenBank蛋白编号BC014638
基因名称 HSF1
Gene ID (NCBI) 3297
RRIDAB_11182610
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ00613
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HSF1 belongs to heat-shock transcription factors that activate heat-shock response genes under conditions of heat or other stresses. Also, HSF1 has been linked with oogenesis, spermatogenesis, and placental development. It can activate AKT and inactivate JNK and CASP3 to protect cardiomyocytes from death. And it has a role in the regulation of life span and establishes a role for SIRT1 in protein homeostasis and heat-shock response. The calculated molecular weight of HSF1 is 57 kDa, but HSF1 migrates at approximately 80 kDa which likely represents different phosphorylation states (PMID: 18434628).